Correlation of Vascular Endothelial Growth Factor Production with Photochemical Reaction-induced Retinal Edema.

Liang Shan; Mi Zheng; Yuan Zhang; Yuan QU; Tian Niu; Qing GU; Kun Liu; Xin Xia

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Correlation of Vascular Endothelial Growth Factor Production with Photochemical Reaction-induced Retinal Edema.

Author: Liang SHAN ^{1
,

2} ; Mi ZHENG ³ ; Yuan ZHANG ¹ ; Yuan QU ⁴ ; Tian NIU ¹ ; Qing GU ⁵ ; Kun LIU ¹ ; Xin XIA ¹ ;
Author Information

1. Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080
2. Shanghai Key Laboratory of Fundus Disease, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China.
3. Department of Ophthalmology, Fujian Provincial Hospital, Fuzhou, Fujian 350004, China.
4. Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China.
5. Shanghai Key Laboratory of Fundus Disease, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China.
Publication Type:Journal Article
MeSH: Angiogenesis Inhibitors; therapeutic use; Animals; Enzyme-Linked Immunosorbent Assay; Erythrosine; toxicity; Fluorescein Angiography; Intravitreal Injections; Macular Edema; chemically induced; drug therapy; metabolism; Ranibizumab; therapeutic use; Rats; Rats, Sprague-Dawley; Recombinant Fusion Proteins; therapeutic use; Tomography, Optical Coherence; Vascular Endothelial Growth Factor A; metabolism
From: Chinese Medical Journal 2016;129(24):2944-2950
CountryChina
Language:English
Abstract:
BACKGROUNDRetinal edema is the major complication of retinal vein occlusion and diabetic retinopathy; it can damage visual function by influencing macular region. This study was to establish a rat retinal edema model and explore the related VEGF expression and observe the responses to anti-VEGF drugs in this model.
METHODSA rat retinal edema model was established by inducing photochemical reaction using a 532 nm laser after the intravenous injection of Erythrosin B. Immediately after the laser treatment, models were given intravitreal injections of Ranibizumab or Conbercept to inhibit VEGF expression, and the changes of retinal thickness were measured. Retinal edema was observed using fundus photography (FP), optical coherence tomography (OCT), and fluoresce in fundus angiography (FFA) at 0, 1, 2, 4, 7 and 14 days after intervention. The retinal VEGF expression was measured using enzyme-linked immunosorbent assay (ELISA) and western blotting at each time point. The rat retinal edema model was also used to verify the function of anti-VEGF polypeptide ZY1.
RESULTSBoth retinal edema and vascular leakage were clearly observed at 1, 2 and 4 days after photochemical induction and the retinal thickness increased notably over the same period. The retinal VEGF expression peaked at day 1 and retina became thickening simultaneously. After the interventions, the VEGF expression of the Ranibizumab and Conbercept groups decreased at each time point compared to the edema group (26.90 ± 3.57 vs. 40.29 ± 6.68, F = 31.269 on day 1 and 22.36 ± 1.12 vs. 29.92 ± 0.93 F = 163.789 on day 2, both P < 0.01); the mean RT (278 ± 4 vs. 288 ± 3, F = 134.190 on day 1 and 274 ± 7 vs. 284 ± 6, F = 64.367 on day 2, both P < 0.05) and vascular leakage in these groups also decreased. The same results were observed in the ZY1 group, particularly at day 2 (P < 0.05).
CONCLUSIONSThis retinal edema model induced by a photochemical reaction is reliable and repeatable. Induced edema increases expression of VEGF. This model can be used to test new drugs.