The surface modification of eguus asinus augment fibroblast adhesion and proliferation on silk fibroin materials.
- Author:
Xiaojie LIAN
1
;
Song WANG
;
Hesun ZHU
;
Zhen GAO
Author Information
1. School of Material Science and Engineering, Beijing Institute of Technology, Beijing 100081, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Cell Adhesion;
drug effects;
Cell Proliferation;
drug effects;
Drugs, Chinese Herbal;
chemistry;
Fibroblasts;
cytology;
Fibroins;
chemistry;
Mice;
NIH 3T3 Cells;
Surface Properties
- From:
Journal of Biomedical Engineering
2010;27(4):806-810
- CountryChina
- Language:Chinese
-
Abstract:
Eguus asinus is one of the rare Chinese drugs famous for promoting blood circulation. In this experiment, it was employed to modify the silk fibroin (SF) by physical blending. Mouse embryonic fibroblasts NIH-3T3 were seeded on pure and modified SF surfaces. The morphological changes of cell on SF surfaces were characterized by optical microscope images. The cell adhesion diameter, the attachment force (F) of a single cell and cell proliferation on SF surfaces were measured by micropipette aspiration system and MTT assay. The results indicated that the fibroblasts seeded on modified SF had larger cell adhesion area, stronger initial attachment force and higher cell proliferation than did the pure SF. In conclusion, eguus asinus improved the adhesion and proliferation of fibroblasts on SF. The research promoted the further use of Chinese drug eguus asinus in tissue engineering.
