Study on 5-carboxyfluorescein N-succinimidyl ester in detecting the degree of cross-linked allogenic tissue.
- Author:
Jun ZAHNG
1
;
Wenyi LIU
;
Luqi LIU
Author Information
1. Department of Cardiac Surgery, Qianfoshan Hospital, Shandong University, Ji'nan 250012, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Aorta, Thoracic;
drug effects;
transplantation;
Bioprosthesis;
Blood Vessel Prosthesis;
Cross-Linking Reagents;
pharmacology;
Esters;
Female;
Fluoresceins;
Fluorescence;
Glutaral;
pharmacology;
Male;
Microscopy, Fluorescence;
Rabbits
- From:
Journal of Biomedical Engineering
2010;27(4):816-819
- CountryChina
- Language:Chinese
-
Abstract:
This study was designed to creat a new method for detecting the degree of cross-linked allogenic tissue based on fluorescent technique. The thoracic aorta of New Zealand rabbits were divided randomly into four groups according to the concentration of Glutaraldehyde (GA), which were group A (control group-with no GA), group B (cross-linked with GA of 0.625%), group C (1.25%), and group D (2.5%). Each group was cross-linked with GA and reacted with 5-FAMSE, and then the fluorescence intensity was observed via fluorescence microscopy (analyzed with Image-Pro Plus 6.0, a professional image analysis software). The differences between groups in order of fluorescence intensity were found to be: group A > roup B > group C > group D (P < 0.01). Meanwhile, the tissue proteins extracted from aorta in each group were submitted to conventional polyacrylamide gel electrophoresis (PGE) after being cross-linked with GA; the result from this method was compared with that from the method of 5-FAMSE. In group A, the tissue proteins extracted from the aorta cross-linked with GA were obviously less than those not cross-linked with GA. However, this phenomenom was not clearly seen among the B, C and D groups. Nevertheless, 5-FAMSE can detect the degree of cross-linkage more conveniently and directly.
