In vitro study for detecting the effects of Bazhen decoction on proliferation and activation of T lymphocytes.
- Author:
Xiaoxia LIU
1
;
Xueling WANG
;
Zhenhong LI
;
Yumin CHEN
;
Jianhua CHEN
Author Information
1. Medical College of Hebei University of Engineering, Handan 056029, China. givesunny@163.com
- Publication Type:Journal Article
- MeSH:
Cell Proliferation;
drug effects;
Cells, Cultured;
Drugs, Chinese Herbal;
pharmacology;
Flow Cytometry;
Humans;
Lymphocyte Activation;
drug effects;
immunology;
T-Lymphocytes;
cytology;
immunology
- From:
Journal of Biomedical Engineering
2010;27(4):855-858
- CountryChina
- Language:Chinese
-
Abstract:
This study was designed to detect the effects of Bazhen decoction on the proliferation and activation of T lymphocytes. T lymphocytes were isolated. The effects of Bazhen decoction on the prolifertion and activation of T lymphocytes, and on the secretion of IFN-gamma IL-2 from T lymphocytes, were detected by MTT, Flow cytometry and ELISA. Results showed that proliferation of T lymphocytes was promoted significantly by different concentration of Bazhen decoction; and after different time, the relationships of "the longer the time, the higher the concentration, and the more enhanced the proliferation" came to be apparent. After 72h, T lymphocytes were activated with different concentration of Bazhen decoction, the rate of CD69+ T cells increased signficantly, the secretion of IFN-gamma and IL-2 increased signficicantly, and the effect exhibited a dose-dependent manner. The results of this epxeriment indicated that Bazhen decoction could promote the proliferation and activation of T lymphocytes.
