Fabrication of alginate microsphere for controlled release and investigation of its release characteristics in vitro.
- Author:
Qingyi HE
1
;
Lisheng WEN
;
Qiang LI
;
Jianzhong XU
;
Fei LUO
Author Information
1. Department of Orthopedic, Southwest Hospital, Third Military Medical University, Chongqing 400038, China. hqy1225@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
Alginates;
administration & dosage;
chemistry;
Animals;
Cattle;
Delayed-Action Preparations;
chemical synthesis;
Drug Carriers;
Glucuronic Acid;
administration & dosage;
chemistry;
Hexuronic Acids;
administration & dosage;
chemistry;
Microspheres;
Serum Albumin, Bovine;
administration & dosage
- From:
Journal of Biomedical Engineering
2007;24(6):1301-1304
- CountryChina
- Language:Chinese
-
Abstract:
This study sought to producte alginate sodium microsphere for controlled release bovine serum albumin(BSA) and to investigate the protein release profile of the BSA-alginate sodium microsphere in vitro, which threw some light on the angiogenesis of tissue engineering bone with vascular endothelial growth factor (VEGF) controlled release under stress. The BSA-alginate sodium microsphere was fabricated with W/O emulsification and ion cross-linking method using alginate sodium. The appearance, microsphere diameter and envelopment rate were detected, and the release characteristics of the BSA-alginate sodium microsphere in vitro was investigated. The alginate microsphere was found to be spherical in shape and evenly distributed. Its mean grain diameter was determined to be 230 +/- 60 microm, carrying capacity 80.3 microg/mg and envelopment rate 61%. Smooth controlled release in BSA-alginate sodium microsphere was shown to last more than 2 weeks. Alginate sodium proved an excellent biodegradable material for protein or polypeptide controlled release. The emulsification and ion cross-linking method was noted to be simple; it was propitious to the structural and functional stablility of protein or polypeptide, thus leading to the prolonged efficacious time of the microsphere.
