Investigation of canine mesenchymal stem cells differentiation to vascular endothelial cell in vitro.
- Author:
Qinggang LI
1
;
Xiuhong XU
;
Zhigang WANG
;
Wenhu LIU
;
Zhexian LI
Author Information
1. Department of Nephrology, Beijing Friendship Hospital Affliating Capital University of Medical Sciences, Beijing 100050, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Bone Marrow Cells;
cytology;
Cell Culture Techniques;
Cell Differentiation;
Cells, Cultured;
Dogs;
Endothelial Cells;
cytology;
Endothelial Growth Factors;
pharmacology;
Mesenchymal Stromal Cells;
cytology;
Vascular Endothelial Growth Factor A;
pharmacology
- From:
Journal of Biomedical Engineering
2007;24(6):1348-1351
- CountryChina
- Language:Chinese
-
Abstract:
To induce endothelial cell, canine bone marrow-derived mesenchymal stem cells (MSCs) were separated from bone marrow by density gradient centrifugation. The isolated MSCs were induced to form endothelial-like cell in the presence of vascular endothelial growth factor (VEGF), endothelial growth factor (EGF) and so on. These results showed that the cells uniformly took on a cobblestone morphology under the light microscope, and cell nucleolus was in the middle of the cells. The cells displayed Weibel-Palade bodies under the transmission electron microscope. vWF, a specific marker of endothelial cell was positive in the cells. The above results demonstrate that MSCs may be differentiated into endothelial cells in vitro.
