Research of GFP-expressing H22 cells and S180 cells by electroblot in vitro and in vivo.
- Author:
Fang MA
1
;
Yuan WANG
;
Jie ZHAO
Author Information
1. Department of Histology and Embryology, West China College of Medicine, Sichuan University, Chengdu 610041, China. mafangmed@126.com
- Publication Type:Journal Article
- MeSH:
Animals;
Electroporation;
Green Fluorescent Proteins;
genetics;
metabolism;
Liver Neoplasms, Experimental;
genetics;
metabolism;
Mice;
Microscopy, Fluorescence;
Sarcoma 180;
genetics;
metabolism;
Transfection;
methods;
Tumor Cells, Cultured
- From:
Journal of Biomedical Engineering
2008;25(1):83-87
- CountryChina
- Language:Chinese
-
Abstract:
Mouse hepatoma H22 cells and sarcoma cells (H22 and S180) were infected with EGFP-N1 by electroblot, and the acquired gfp-H22 and gfp-S180 cells expressing strong green fluorescence protein (GFP) fluorescence were supplemented with medium G418 Sigma (800 mg/ml). Meanwhile, the models bearing cancer (gfp H22 and gfp S180) subcutaneously and with abdominal cavity were established. There were no statistically significant differences by comparison on the cell phenotype, ultramicrostructure, growth curve and bearing cancer time between the H22 cells and S180 cells (P>0.05). The GFP fluorescence was detected with whole body GFP imaging system in vivo and with fluorescence microscope. According to the results of in vitro and in vivo assay, it was shown that, by application of fluorescence technology, the GFP-expressing H22 cells and S180 cells could be used in further studies on the tumor biological behavior.
