Heat-shock protein 27 linked to multi-drug resistance in human hepatic cancer cell lines.
- Author:
Shi-fang PENG
1
;
Lei FU
;
De-ming TAN
Author Information
- Publication Type:Journal Article
- MeSH: Drug Resistance, Multiple; Drug Resistance, Neoplasm; HSP27 Heat-Shock Proteins; metabolism; Hep G2 Cells; Humans; Liver Neoplasms; metabolism
- From: Chinese Journal of Hepatology 2007;15(5):362-365
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEA proteomics approach was applied for finding multi-drug resistance (MDR) related proteins in hepatic cancer cell lines.
METHODSTwo-dimensional electrophoresis (2-DE) was used to separate the total proteins of vincristine-resistant hepatic cancer cell line HepG2/VCR and its counterpart HepG2. The differential expression proteins between the two cell lines were identified by both MALDI-TOF-MS and ESI-Q-TOF-MS. Heat-shock protein 27 (HSP27), one of the differential expression proteins in the development of MDR of HepG2/VCR, was analyzed by HSP27 antisense oligonucleotides (ASOs).
RESULTSAs a high expression protein in HepG2/VCR, HSP27 was identified, and the suppression of HSP27 expression by HSP27 ASO enhanced the vincristine chemosensitivity of HepG2/VCR (P less than 0.05).
CONCLUSIONHSP27 is linked to MDR in human hepatic cancer cell line HepG2/VCR.
