The expression of peroxiredoxin II in hepatocellular carcinoma and its significance.
- Author:
Hai-ying YUE
1
;
Ji CAO
;
Ji-feng CUI
;
Zhi DAI
;
Jian-jia SU
;
Xiao-xian DUAN
;
Chun YANG
;
Hui-fen YUE
;
Yuan LI
;
Yin-kun LIU
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Animals; Carcinoma, Hepatocellular; metabolism; pathology; Female; Humans; Liver; metabolism; pathology; Liver Neoplasms; metabolism; pathology; Liver Neoplasms, Experimental; metabolism; pathology; Male; Middle Aged; Peroxiredoxins; genetics; Tupaiidae
- From: Chinese Journal of Hepatology 2007;15(5):366-369
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the mRNA and protein expressions of peroxiredoxin II (PrxII) in hepatocellular carcinoma (HCC) and their significance.
METHODSHCC was induced by aflatoxin B1 (AFB1) in 6 tree shrews (Tupaia belangeri chinensis). The expression levels of PrxII mRNA and protein were detected by reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot on HCC tissues and on their surrounding liver tissues (para-HCC). Biopsied liver tissues were taken before the HCC induction (pre-HCC) from the same animals and from a group of blank controlled animals that served as controls. Liver biopsy specimens from 18 cases of human HCC and from 17 healthy human volunteers were studied using the same methods.
RESULTSThe mRNA and protein expressions of PrxII in tree shrew HCC tissues were significantly higher than those in para-HCC and pre-HCC tissues, and also higher than those in the liver tissues from the control animals (all P < 0.05). The expression levels of PrxII mRNA and protein in human HCC tissues were also significantly higher than those in their para-HCC tissues and in the human normal liver tissues (P < 0.05).
CONCLUSIONPrxII might play an important role in hepatocarcinogenesis and might be used as a molecular target for HCC prevention and treatment.
