Separation and identification of the exosomes derived from a mouse hepatoma carcinoma cell line (H22) and initial investigation of their protein composition.
- Author:
Jing LI
1
;
Yi SHEN
;
Wei-xue TANG
;
Li CHEN
;
Hong DUAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Carcinoma, Hepatocellular; immunology; metabolism; Cell Line, Tumor; Exosomes; immunology; secretion; Histocompatibility Antigens Class II; immunology; Liver Neoplasms; immunology; metabolism; Mice; Peptide Mapping
- From: Chinese Journal of Hepatology 2007;15(6):437-440
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo separate and identify the exosomes derived from a mouse hepatoma carcinoma cell line (H22) and to detect their protein composition, and to investigate the possibility of using these exosomes as a kind of tumor vaccine.
METHODSExosomes were purified by serial ultracentrifugation and sugar density ultracentrifugation, and then they were observed and identified by electron microscopy. Exosomes underwent peptide mass fingerprint and Western blot analyses.
RESULTSH22 cell-derived exosomes were 20-90 nm round or oval vesicles. The exosomes expressed HSP70, ICAM-1, EF-G2, DLC-A, C-myc protein and Vav-2 protein.
CONCLUSIONSerial ultracentrifugation and sugar density ultracentrifugation can be used to purify H22 cell-derived exosomes. H22 cell-derived exosomes express a distinct set of proteins involving in and/or relating to antigen presentation (HSP70, ICAM-1), migration (DLC-A), adhesion (ICAM-1), cytoskeleton (EF-G2) and tumour antigens (C-myc, Vav-2). The exosomes have immunogenicity.
