Prenatal Diagnosis of Duchenne Muscular Dystrophy (DMD) by Multiplex Polymerase Chain Reaction (PCR) with Chorionic Villi.
- Author:
Kyo Won LEE
;
Seon Woong YOON
;
Woo Sup CHANG
;
Jin Yeong KIM
;
Sook Hwan LEE
;
Sang Joon LEE
;
Sung Do KIM
;
Jong Sul HAN
- Publication Type:Original Article
- MeSH:
Chorion*;
Chorionic Villi*;
DNA;
Exons;
Humans;
Multiplex Polymerase Chain Reaction*;
Muscular Dystrophy, Duchenne*;
Polymerase Chain Reaction;
Preimplantation Diagnosis;
Prenatal Diagnosis*
- From:Korean Journal of Obstetrics and Gynecology
1999;42(9):2028-2032
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: To evaluate the efficacy of prenatal multiplex PCR with chorionic villi, in the case of family history of DMD due to exon deletion. METHODS: DNA was extracted when cells' size are 1x 106 on T- flask surface area reach to 25 cm2 after 2 weeks from sex confirmation. Average DNA concentration was 50-100 ng and multiplex PCR test was performed from DNA extraction. RESULT: PCR was done for 17 exons devided into 4 groups. Seventeen exons were all amplified with their right size. CONCLUSIONS: This method is DNA analysis for prenatal diagnosis of DMD with chorionic villi in the family of DMD. This is useful when preimplantation genetic diagnosis is not available.
