Influence of Interferon alpha-2b on proliferation inhibition and apoptosis induction in HL-60 cells.
- Author:
Hong YU
1
;
Li-Rong SUN
;
Xiu-Ying PANG
;
Xue-Rong LI
;
Yuan LU
;
Ai-Qin SONG
Author Information
1. Department of Pediatrics, Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, China.
- Publication Type:Journal Article
- MeSH:
Antineoplastic Agents;
pharmacology;
Apoptosis;
drug effects;
CD13 Antigens;
biosynthesis;
genetics;
Cell Proliferation;
drug effects;
HL-60 Cells;
Humans;
Interferon-alpha;
pharmacology;
Recombinant Proteins
- From:
Journal of Experimental Hematology
2007;15(1):56-58
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effects of interferon alpha-2b on proliferation and apoptosis in HL-60 cells, HL-60 cells were cultured in different concentrations of IFN alpha-2b. The morphologic changes were observed by Wright's and acridine orange (AO) and ethidium bromide (EB) staining respectively. Inhibition of proliferation was detected by MTT. Expression of CD13(+) was checked by indirect fluoroimmunoassay. The results showed that apoptosis rate of HL-60 cells assayed by the above-mentioned two methods was (51 +/- 2)% and (78 +/- 3)% respectively and OD(570) values of proliferation inhibited were 1.8 +/- 0.1 and 1.0 +/- 0.1 respectively when the concentrations of the IFN(alpha-2b) were 500 and 10,000 U/ml in culture for 48 hours. Morphology and count of CD13(+) cells were changed. CD13(+) cell expression rate was (62 +/- 2)% and (30 +/- 3)% respectively when the concentrations of the IFN(alpha-2b) were 500 and 10,000 U/ml in culture for 48 hours. It is concluded that IFN(alpha-2b) can enhance the apoptosis of HL-60 cells, inhibit their proliferation, promote their maturation and differentiation.
