Apoptosis of HL-60 cells induced by recombinant common Buckwheat trypsin inhibitor.
- Author:
Li GAO
1
;
Yu-Ying LI
;
Zheng ZHANG
;
Zhuan-Hua WANG
;
Hong-Wei WANG
;
Li ZHANG
;
Lei ZHU
Author Information
1. Key Laboratory for Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan 030006, China.
- Publication Type:Journal Article
- MeSH:
Antineoplastic Agents;
pharmacology;
Apoptosis;
drug effects;
Cell Proliferation;
drug effects;
Fagopyrum;
chemistry;
HL-60 Cells;
Humans;
Recombinant Proteins;
biosynthesis;
genetics;
pharmacology;
Trypsin Inhibitors;
biosynthesis;
genetics;
pharmacology
- From:
Journal of Experimental Hematology
2007;15(1):59-62
- CountryChina
- Language:Chinese
-
Abstract:
The study was purposed to investigate the apoptosis of HL-60 cells induced by recombinant common buckwheat trypsin inhibitor (rBTI) and its mechanism. The inhibition rate of rBTI on HL-60 cells was detected by MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide); the morphology of HL-60 nuclei was observed by fluorescence microscopy; the apoptosis cells of HL-60 detected by agarose gel electrophoresis and the changes of apoptosis rate was assayed by flow cytometry (FCM), when the HL-60 cells were treated with different concentration of rBTI for 24 hours. The results showed that the growth of HL-60 cells was inhibited evidently after treatment with rBTI in a dose-dependent manner, but there were minimal effects on normal human peripheral blood mononuclear cells (PBMNCs). The nuclei of HL-60 cells showed the characteristics of apoptosis, the analysis by flow cytometry indicated that the apoptosis rate of HL-60 cells was 52% after treatment with rBTI (100 microg/ml), DNA analyzed by agarose gel electrophoresis showed "ladder" pattern. It is concluded that rBTI obviously inhibits growth of HL-60 and induces its apoptosis which provides a foundation for use of recombinant common buckwheat trypsin inhibitor to cure the acute myeloid leukemia.
