Effect of nimodipine on mechanisms of HL-60 cell apoptosis induced by cytarabine.
- Author:
Li-Rong SUN
1
;
Bin-Chang GAO
;
Xiu-Ying PANG
;
Yuan LU
;
Xue-Rong LI
;
Ai-Qin SONG
Author Information
1. Department of Pediatrics, Affiliated Hospital of Qingdao Medical College, Qingdao University, Qingdao 266003, China. sunlr@vip.sina.com
- Publication Type:Journal Article
- MeSH:
Antimetabolites, Antineoplastic;
pharmacology;
Apoptosis;
drug effects;
Cytarabine;
pharmacology;
Drug Resistance, Neoplasm;
drug effects;
Drug Synergism;
Gene Expression Regulation, Neoplastic;
HL-60 Cells;
Humans;
Nimodipine;
pharmacology;
Proto-Oncogene Proteins c-bcl-2;
biosynthesis;
genetics;
bcl-X Protein;
biosynthesis;
genetics
- From:
Journal of Experimental Hematology
2007;15(1):72-75
- CountryChina
- Language:Chinese
-
Abstract:
The aim was to study the mechanisms of HL-60 cell apoptosis induced by nimodipine (NMDP) and cytarabine (Ara-C). The DNA fragment was detected by agarose gel electrophoresis. The expressions of bcl-2 and bax gene proteins related with apoptosis were investigated by immunohistochemistry. The results showed that HL-60 cell apoptosis rate had been increasing in the experimental groups compared with the control group since culturing 8 hours. The expression of Bcl-2 protein was lower and the expression of Bax protein was higher in the experimental groups than that in the control group, while ratio of bcl-2/bax was lower in the experimental groups than that in the control group. It is concluded that NMDP and Ara-C induce apoptosis of HL-60 cells, and the mechanism of apoptosis induced by them may down-regulate the expression of bcl-2 gene and up-regulate the expression of bax gene. The mechanism of HL-60 cell apoptosis induced by Ara-C and NMDP is probably associated with the down-regulation of Bcl-2 protein expression.
