Role of gammadeltaT cells in pathogenesis of acquired pure red cell aplastic anemia.
- Author:
Min LIU
1
;
Ting LIU
;
Wen-Tong MENG
;
Huan-Ling ZHU
;
Xu CUI
Author Information
1. Department of Hematology, Hematological Research Laboratory, West China Hospital of Sichuan University, Chengdu 610041, China.
- Publication Type:Journal Article
- MeSH:
Adult;
Aged;
CD4-CD8 Ratio;
Cells, Cultured;
Cyclosporine;
therapeutic use;
Female;
Flow Cytometry;
Humans;
Immunosuppressive Agents;
therapeutic use;
Male;
Middle Aged;
Receptors, Antigen, T-Cell, gamma-delta;
physiology;
Red-Cell Aplasia, Pure;
drug therapy;
etiology;
immunology;
T-Lymphocyte Subsets;
cytology;
T-Lymphocytes;
cytology;
T-Lymphocytes, Cytotoxic;
immunology
- From:
Journal of Experimental Hematology
2007;15(1):142-146
- CountryChina
- Language:Chinese
-
Abstract:
This study was purposed to investigate the changes in quantum and function of gammadelta T cell subsets, and to explore its significance in pathogenesis of acquired pure red cell aplastic anemia (A-PRCA). Eleven patients were diagnosed as A-PRCA based on bone marrow smear and biopsy, and were treated with cyclosporine A and glucosidorum tripterygll totorum. The flow cytometry technique was used for analyses of T cells subsets and gammadelta T cells. Furthermore, peripheral mononuclear cells (MNC) isolated from A-PRCA patients were cultured in RPMI 1640 medium (10(5) cells/ml) containing 10% FCS, phytohemagglutinin (PHA, 10 microg/ml), and recombinant human interleukin-2 (rIL-2, 50 U/ml) for two weeks, then gammadelta T cells were isolated with the TCRgammadelta Microbead Kit from cultured cells. The collected gammadelta T cells were incubated with normal control bone marrow MNC in RPMI 1640 medium (37 degrees C, 5% CO2 atmosphere) for CFU-E, CFU-GM, and BFU-E colony assay. The result showed that compared with the control group, CD3(+), CD8(+) cells increased significantly in the patient group (P < 0.05), the CD4(+)/CD8(+) ratio decreased and reversed, and gammadelta T cells were significantly increased in patient group (P < 0.05). After treatment with cyclosporine A, 9 out of 11 patients got good response, and CD3(+), CD8(+) cells in the responding patient decreased, the ratio of CD4(+)/CD8(+) returned to normal, and gammadelta T cells also decreased to normal range. Moreover, in vitro culture, the gammadelta T cells isolated from A-PRCA patients showed an inhibiting action to CFU-E and BFU-E but not to CFU-GM in a dose-dependent manner. It is concluded that gammadelta T cells increase in A-PRCA patients, and decrease in parallel to normal range with significant improvement of anemia symptoms after immune suppressive therapy. The gammadelta T cells isolated from A-PRCA patients showed an inhibiting action to CFU-E and BFU-E but not to CFU-GM in vitro culture, suggesting that gammadelta T cells may bring an impact on the research of A-PRCA pathogenesis. Cyclosporine A demonstrated better therapeutic effect on A-PRCA patients.
