Influence of rhG-CSF on activity of sphingosine kinase in monocytes.
- Author:
Wen-Rong HUANG
1
;
Li-Sheng WANG
;
Hai-Feng DUAN
;
Chun-Ji GAO
;
Zhuo-Zhuang LU
;
Hua WANG
;
Wan-Ming DA
Author Information
1. Department of Hematology, PLA General Hospital, Beijing 100853, China.
- Publication Type:Journal Article
- MeSH:
Granulocyte Colony-Stimulating Factor;
pharmacology;
Hematopoietic Stem Cell Mobilization;
Humans;
Monocytes;
cytology;
enzymology;
Phosphotransferases (Alcohol Group Acceptor);
drug effects;
metabolism;
Receptors, Granulocyte Colony-Stimulating Factor;
biosynthesis;
genetics;
Recombinant Proteins
- From:
Journal of Experimental Hematology
2007;15(1):156-159
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this research was to understand the influence of rhG-CSF on the sphingosine kinase (SphK) activity of monocytes. The peripheral blood monocytes were collected from 6 peripheral blood progenitor cell donors on the fifth day of mobilization with rhG-CSF and from 5 blood donors' buffy coats. The mRNA expressions of monocyte G-CSF receptor and SphK were tested with RT-PCR. The changes of SphK activity of monocytes were assayed after being treated with rhG-CSF. The results showed that the two kinds monocytes collected from both blood donors and peripheral blood progenitor cell donors mobilized with rhG-CSF expressed mRNA of G-CSF receptor and SphK. The SphK activity of monocytes collected from blood donors was not changed significantly after being treated with rhG-CSF (P > 0.05). The SphK activity of monocytes collected from peripheral blood progenitor cell donors transiently increased by (39.6 - 87.2)% after being treated by means of rhG-CSF (P < 0.05) without obviously dose-dependent effect. It is concluded that the SphK activity of monocytes collected from peripheral blood progenitor cell donors can be activated by rhG-CSF.
