Effects of NKG2D and its ligands RAE-1 and H60 on graft-versus-tumor response.
- Author:
Xiao-Feng LI
1
;
Qiang CHEN
;
Yun-Bin YE
;
Lan-Feng FAN
;
Ming-Shui CHEN
;
Jie-Yu LI
;
Hui-Qing CHEN
;
Shu-Ping CHEN
;
Zhi-Feng ZHOU
Author Information
1. Department of Oncology, Quanzhou Hospital for Traditional Chinese Medicine, Quanzhou 362000, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Female;
Graft vs Leukemia Effect;
drug effects;
immunology;
Hematopoietic Stem Cell Transplantation;
Killer Cells, Natural;
drug effects;
immunology;
Leukemia, Experimental;
immunology;
therapy;
Ligands;
Male;
Mice;
Mice, Inbred BALB C;
Mice, Inbred C57BL;
Mice, Inbred Strains;
Minor Histocompatibility Antigens;
biosynthesis;
genetics;
NK Cell Lectin-Like Receptor Subfamily K;
Nuclear Matrix-Associated Proteins;
biosynthesis;
genetics;
Nucleocytoplasmic Transport Proteins;
biosynthesis;
genetics;
Receptors, Immunologic;
blood;
genetics;
Receptors, Natural Killer Cell
- From:
Journal of Experimental Hematology
2007;15(1):160-164
- CountryChina
- Language:Chinese
-
Abstract:
The study was purposed to explore the effects of NKG2D receptor and its ligands RAE-1 and H60 on graft-versus-tumor (GVT) response induced by MHC haploidentical bone marrow/spleen cell transplantation. Female (BALB/c x C57BL/6) F1 mice (CB6F1, H-2K(b/d)) inoculated with H22 cells to develop a solid tumor model were the recipients, and bone marrow mixed with spleen cells of the healthy male C57BL/6 (H-2K(b)) mice were the donor cells. GVT response was observed after transplantation that from donor cells T and NK cells were purged with anti-CD3 and anti-NK monoclonal antibody, and the NKG2D receptor was blocked with anti-NKG2D monoclonal antibody, the expression levels of RAE-1 and H60 mRNA in tumor tissue were measured by means of semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) at different time points after transplantation. The results showed that the GVT response of transplantation was reduced after in vitro depletion of T and NK cells or blocking NKG2D receptor in donor cells of the graft, the expression levels of RAE-1 and H60 mRNA in tumor tissue increased after transplantation of haploidential bone marrow mixed with spleen cells. It is concluded that NKG2D and its ligands RAE-1 and H60 may play important roles in GVT response.
