Inhibition of leukemic cell proliferation by human soluble VEGF-R1.
- Author:
Jing ZHU
1
;
Yan-Qiu SONG
;
Wei LI
;
Guan-Jun WANG
Author Information
1. Department of Hematology and Oncology, The First Hospital, Jilin University, Changchun 130021, China.
- Publication Type:Journal Article
- MeSH:
Cell Proliferation;
drug effects;
HL-60 Cells;
Humans;
K562 Cells;
RNA, Messenger;
biosynthesis;
genetics;
U937 Cells;
Vascular Endothelial Growth Factor A;
antagonists & inhibitors;
biosynthesis;
genetics;
Vascular Endothelial Growth Factor Receptor-1;
biosynthesis;
genetics;
physiology
- From:
Journal of Experimental Hematology
2007;15(1):168-174
- CountryChina
- Language:Chinese
-
Abstract:
The current study was purposed to investigate the inhibitory effect of human soluble vascular endothelial growth factor-1 (sFLT-1) on the proliferation of leukemic cells in vitro. Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the VEGF mRNA and VEGF-R1 (FLT-1) mRNA in K562, HL60, U937 leukemic cell lines and bone marrow LTC-IC. Flow cytometry was used to detect the VEGF and VEGF-R1 (FLT-1) in all above-mentioned cells. VEGF concentrations in the cell culture supernatants were determined by enzyme-linked immunosorbent assay (ELISA). Cell proliferation was determined by MTT after adding sFLT-1 to K562, HL60 and LTC-IC culture system. The result showed that expression of VEGF could be detected in K562, HL60, U937 leukemic cell lines and LTC-IC, especially K562, K562 and HL60 cell lines also expressed FLT-1, but a little expression was found in U937 and LTC-IC. sFLT-1 could effectively inhibit the growth of K562 and HL60 cell lines in dose-dependent manner. The highest inhibition rate was found at 48 hours after adding sFLT-1. It is concluded that sFLT-1 can inhibit the growth of some leukemic cell lines, and the inhibition effect enhances as the concentration of the sFLT-1 increase, but sFLT -1 not influence the proliferation of normal marrow cells.
