Effects of novel human chemokine-like factor superfamily 8 on proliferation and EGFR expression of HL-60 cells.
- Author:
Shao-Qian CHEN
1
;
Ying DU
;
Yu-Min HUANG
;
Xin WANG
;
Qiao-Li GU
;
Zi-Ming DONG
Author Information
1. Department of Hematology, The First Affiliated Hospital, Zhengzhou University, Zhengzhou 450055, China.
- Publication Type:Journal Article
- MeSH:
Cell Proliferation;
Chemokines;
metabolism;
Gene Expression Regulation, Neoplastic;
HL-60 Cells;
Humans;
MARVEL Domain-Containing Proteins;
RNA, Messenger;
metabolism;
Receptor, Epidermal Growth Factor;
metabolism;
Transfection
- From:
Journal of Experimental Hematology
2007;15(3):458-461
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the effect of chemokine-like factor superfamily 8 (CKLFSF8) on proliferation and expression of epidermal growth factor receptor (EGFR) of HL-60 cells. Expression of CKLFSF8 mRNA on HL-60 cell line was assayed by RT-PCR; the target gene was transfected into the cells by lipid vector, cell proliferation was determined by MTT assay, while expression of EGFR in HL-60 was determined by immunocytochemical technique. The results indicated that expression of CKLFSF8 existed in HL-60 cells. After transfection, cell proliferation was inhibited (P < 0.05) and the expression of EGFR in HL-60 cells was also discovered to be inhibited (P < 0.05). It is concluded that the proliferation and expression of EGFR in HL-60 cells can be inhibited by transfection of CKLFSF8. The novel chemokine may provide a new approach in the treatment of leukemia.
