Differentiation of HL-60 cells directly cocultured with HFCL cells and alteration of their gene expression profile.
- Author:
Rong LIANG
1
;
Gao-Sheng HUANG
;
Xie-Qun CHEN
;
Qing-Xian BAI
;
Zhe WANG
;
Bao-Xia DONG
;
Wen-Qing WANG
;
Wei-Ping ZHANG
Author Information
1. Department of Hematology, Xijing Hospital, The Fourth Military Medical University, Xi'an 710033, China.
- Publication Type:Journal Article
- MeSH:
Cell Transformation, Neoplastic;
genetics;
Coculture Techniques;
Fibroblasts;
cytology;
physiology;
Gene Expression;
Gene Expression Profiling;
HL-60 Cells;
Humans;
Stromal Cells;
cytology;
physiology
- From:
Journal of Experimental Hematology
2007;15(3):490-495
- CountryChina
- Language:Chinese
-
Abstract:
To study the molecular mechanism of the effect of fibroblastoid stromal cells (HFCL) from human bone marrow on the proliferation and differentiation in acute myeloid leukemia HL-60 cells, the cell cycle was analyzed by flow cytometry (FCM); the cell differentiation was determined by morphology NBT test and flow cytometric detection for expression of CD11b, CD14, CD13 and CD33; the genes differently expressed between HL-60 cells and HL-60 cells directly cocultured with HFCL were detected by using Affymetric oligo microarray technique. The changes of expression in some key genes were confirmed by using RT-PCR and Northern blot. The results showed that the percentage of G(1) phase cells in AML cells cocultured with HFCL cells was higher than that without HFCL cells, and the percentage of S phase cells was lower. The NBT positive cells and the expression of CD11b and CD14 increased. It was found that after direct contact of HL-60 cells with HFCL cells for 96 hours, the expression levels of 582 genes were up-regulated, 1 323 genes down-regulated. It is concluded that many genes may take part in the influence of HFCL cells on HL-60 cells, which may give important insights into the important molecules and pathways or cross-talk involved in the interaction between the AML cells and stromal cells.
