Effects of Yiqi Jiangzhuo Decoction on transforming growth factor-beta1 mRNA expression in treating ventricular remodeling in mice with repetitive CVB3m infection caused viral myocarditis.
- Author:
Ying-wei WANG
1
;
Ling GUO
;
Qi-hong LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Coxsackievirus Infections; drug therapy; Drugs, Chinese Herbal; therapeutic use; Enterovirus B, Human; drug effects; Gene Expression; drug effects; Male; Mice; Myocarditis; drug therapy; pathology; virology; Phytotherapy; RNA, Messenger; biosynthesis; genetics; Recurrence; Reverse Transcriptase Polymerase Chain Reaction; Transforming Growth Factor beta1; genetics; Ventricular Remodeling; drug effects; genetics; physiology
- From: Chinese Journal of Integrated Traditional and Western Medicine 2006;26(4):341-343
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of Yiqi Jiangzhuo Decoction (YJD) on transforming growth factor-beta1(TGF-beta1) mRNA expression in treating ventricular remodeling in viral myocarditis.
METHODSAfter being established into chronic viral myocarditis model by 4 times repetitively infecting with gradient multiplicative CVB3m, 160 male mice were divided into the blank group, the model group, the TCM treated group and the Western medicine treated group. On the 10th, 30th and 60th day after the last time of infection, mice were killed to observe their pathological changes of myocardium with HE staining and to detect TGF-beta1 mRNA expression in myocardial tissue with semi-quantitative RT-PCR method.
RESULTSPathological changes of myocardium alleviated, and TGF-beta1 mRNA expression distribution area reduced significantly in the two treated groups, as compared with those in the model group (all P < 0.05).
CONCLUSIONYJD could inhibit the hyperplasia and reconstruction of ECM by down-regulating TGF-beta1 mRNA expression to improve cardiac pathological changes in myocarditis, so as to prevent the occurrence of ventricular remodeling and the conversion of disease to dilative cardiomyopathy.
