Effect of some active Chinese herbal fraction on brain tissue proteomic profile of ischemic mouse.
- Author:
Yuan-ying SONG
1
;
Zhong WANG
;
Xun QU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Brain; metabolism; Brain Infarction; drug therapy; metabolism; Drugs, Chinese Herbal; chemistry; therapeutic use; Female; Male; Mice; Phytotherapy; Protein Array Analysis; Proteomics; methods; Reperfusion Injury; drug therapy; metabolism; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; methods
- From: Chinese Journal of Integrated Traditional and Western Medicine 2006;26(6):526-528
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of some active Chinese herbal fraction on protein expression of brain tissue in ischemic mouse with proteomic technique.
METHODSIschemia-reperfusion mice were treated with baicalin, geniposide, cholic acid and concha margaritifera respectively for 3 hrs, and then their brain tissue were taken to extract the total protein. Protein expression in ischemic mouse brain was analyzed with surface-enhanced laser desorption/inionation-time of flight-mass spectra (SELDI-TOF-MS) protein-chip.
RESULTSThe four components tested had effect on 3 target proteins at 5373Da, 5707Da and 15103Da, showing the nature of multi-target and with different action on protein expression.
CONCLUSIONProtein-chip is an effective approach for exploring the pharmacological mechanism of Chinese herbal fraction.
