Effect of maixinkang capsule on Ca2+ and mitochondrial membrane potential in liver cells of ApoE(-/-) mice.
- Author:
Guang-juan ZHENG
1
;
Wen-gao ZHANG
;
Qing-jun ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Animals, Newborn; Apolipoproteins E; genetics; Calcium Channels; drug effects; Hepatocytes; physiology; Membrane Potentials; Mice; Mice, Knockout; Mitochondrial Membranes; physiology
- From: Chinese Journal of Integrated Traditional and Western Medicine 2006;26(5):427-430
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effect of Maixinkang Capsule (MXK) on Ca2t concentration and mitochondrial membrane potential in liver cells of ApoE(-/-) mice.
METHODSLiver cells from ApoE(-/-) mice were separated using collagenase digestive method. After the primary cells were cultured for 8 days in vitro, the concentration of 10% MXK contained rat's serum was added into the culture fluid. The Ca2+ concentration and mitochondrial membrane potential in liver cells after 48-hr culture were measured by confocal laser scanning microscopy with Flou-3 and Jc-1 as probes.
RESULTSMXK could decrease Ca2+ concentration in liver cells, which was significantly different to that in the control group (P < 0.01). Meanwhile, MXK could significantly improve mitochondrial membrane potential in liver cells (P < 0.01). There was no obvious dose-effect relationship shown in both effects of MXK.
CONCLUSIONMXK can decrease Ca2+ concentration and improve the mitochondrial membrane potential in liver cells of ApoE(-/-) mice so as to regulate the lipids and prevent the occurrence and development of hyperlipemia and atherosclerosis.
