Effect of jiangu granule on levels of urinary deoxypyridinoline and osseous intigrin beta1 mRNA expression in Ovariectomized osteoporosis model rats.
- Author:
Yan-ping LIN
1
;
Rui-xiang ZHOU
;
Yi-long LI
Author Information
- Publication Type:Journal Article
- MeSH: Amino Acids; urine; Animals; Drugs, Chinese Herbal; pharmacology; Female; Nerve Tissue Proteins; metabolism; Osteoblasts; metabolism; Osteogenesis; drug effects; Osteoporosis; metabolism; prevention & control; Ovariectomy; RNA, Messenger; biosynthesis; genetics; Rats; Rats, Sprague-Dawley
- From: Chinese Journal of Integrated Traditional and Western Medicine 2006;26(5):435-438
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the regulatory effect of Jiangu Granule (JGG) on osteoblast and its effect on bone absorption on the molecular level and from aspect of bone metabolism.
METHODSOsteoporosis model SD rats established by ovariectomy were interfered by drug. Levels of urinary deoxypyridinoline (D-Pyr) and osseous integrin beta1 (Itgbeta1) mRNA expression were detected by ELISA and in situ hybridization, respectively.
RESULTSLevel of urinary D-Pyr increased (P < 0.01) and osseous Itgbeta1 mRNA expression decreased significantly in the model rats. After treatment with JGG for 12 weeks, the D-Pyr levels in them decreased significantly (P < 0.05), while the quantity of osteoblasts and the expression of Itgbeta1 mRNA in bone tissue increased obviously. Conclusion JGG can not only decrease the catabolism of collagen fiber type I in bone matrix, but also promote the osteogenic activity of osteoblast. So, it can improve or reverse the pathological tendency of osteogenesis shortage caused by E2 reduction to play its action in strengthening bone.
