Study on genetic-chemical relation of Pterocephali Herba based on DNA barcode and UFLC.

Author: Yu-Xin ZHANG ¹ ; Cong-Yin LI ¹ ; Chuan LIU ¹ ; Jiang XU ² ; Li XIANG ² ; Gang FAN ¹ ; Yi ZHANG ¹
Author Information

1. College of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
2. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Publication Type:Journal Article
Keywords: DNA barcoding; Pterocephalus hookeri; Tibetan medicine; UFLC; genetic-chemical; quality evaluation; relation
From: China Journal of Chinese Materia Medica 2016;41(4):572-577
CountryChina
Language:Chinese
Abstract: DNA barcoding technique in combination with UFLC analysis technology was used to evaluate the quality of Tibetan medicine Pterocephalus hookeri from species identification and chemical qualitative and other aspects. Hybrid identification was established by DNA barcoding; UFLC-PDA was adopted to analyse fingerprint of different parts of Pterocephali Herba, and SPSS and Grey relation software were used for data analysis. The result showed that DNA barcoding is an accurate and reliable method in origin identification of Pterocephalus hookeri. The compounds in overground is more than underground by analysis of the different part fingerprint by UFLC. The genetic gene may be involved in the secondary metabolites of iridoid glycosides. Pertinence between gene and chemical component, as a new model established, could be suited for quality evaluation and resources protection.