Expression of key enzyme genes and content of saikosaponin in saikosaponin biosynthesis under drought stress in Bupleurum chinense.
- Author:
Yu ZHANG
1
;
Zi-Yun ZHOU
1
;
Peng-Guo XIA
2
;
Zong-Suo LIANG
1
;
Shu-Li LIU
3
;
Zhi-Liang LIU
3
Author Information
- Publication Type:Journal Article
- Keywords: Bupleurum chinense; andrographolide; gene expression; saikosaponin
- From: China Journal of Chinese Materia Medica 2016;41(4):643-647
- CountryChina
- Language:Chinese
- Abstract: To research the expression of key enzymes in saikosaponin biosynthesis and the content of saikosaponin under the drought stress, the study focused on the gene-level and the end product responses to environmental change. Taking the five months of Bupleurum chinense as research materials, the contents of saikosaponin A and saikosaponin D under different stress levels were measured by HPLC. The drought was simulated by poly ethylene glycol. The real-time fluorescence quantitative PCR was used to analyze the expression of four key enzymes genes HMGR, IPPI, FPS, β-AS and the expression of β-tubulin was set as a reference gene. The results showed that drought stress significantly improved the content of saikosaponin. The contents of SSa and SSd were highest researching 0.648% and 0.781%, respectively when the concentration of PEG was 10%. Meanwhile, the results reflected that the expression of four key enzymes had risen differently and FPS, β-AS raised significantly(P<0.01). In addition, the results of correlation analysis showed that there was a significant positive correlation between the expression of the four key enzymes genes and the content of saikosaponin. In a word, the contents of secondary metabolites were regulated by the expression of key enzymes genes under the drought stress in B. chinense.
