Screening target genes for bimolecular marking methods of Magnolia quality.

Author: Yu-Yang ZHAO ¹ ; Jian YANG ¹ ; Liang-Ping ZHA ¹ ; Dong-Mei XIE ¹ ; Lu-Qi HUANG ¹ ; Yuan YUAN ¹
Author Information

1. State Key Laboratory of Dao-di Herbs Breeding Base, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Publication Type:Journal Article
Keywords: Magnolia officinalis; bimolecular marking methods; cinnamyl alcohol dehydrogease; lignins
From: China Journal of Chinese Materia Medica 2016;41(4):648-653
CountryChina
Language:Chinese
Abstract: The study used use bimolecular marking methods to evaluate the lignans of Magnolia officinalis and M. officinalis var. biloba. First, we compare the chemical constituents between M. officinalis and M. officinalis var.biloba. There were significant differences in concentration of magnolignan I between leaves of these two varieties. Then we further select the p-hydroxyphenyl lignin to mining the key enzyme genes of biosynthesis from Magnolia transcriptome, and screened an encoding cinnamyl alcohol dehydrogease gene as the candidate marker of bimolecular marking methods of Magnolia quality by comparing of the expression level and structure variation in homologous gene between M. officinalis and M. officinalis var.biloba. The established method provides the technical support for bimolecular marking methods of Magnolia quality evaluation.