Mitogen-activated protein kinase genes of Artemisia annua and their expression analysis under Cadmium stress.

Author: Liang-Yun ZHOU ¹ ; Chao-Geng LV ¹ ; Chuan-Zhi KANG ¹ ; Sheng WANG ¹ ; Jin-Fu TANG ¹ ; Li-Ping KANG ¹ ; Lan-Ping GUO ¹
Author Information

1. State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Publication Type:Journal Article
Keywords: Artemisia annua; Cadmium stress; MAPK gene; expression analysis
From: China Journal of Chinese Materia Medica 2016;41(6):1016-1020
CountryChina
Language:Chinese
Abstract: In order to study Artemisia annua under cadmium stress, whether there are corresponding MAPK genes involved in transduction of the cadmium signal. 17 AaMAPK genes, named AaMAPK1-AaMAPK17 repectively, were finally obtained by using Trinity method for de novo assembly of transcripts from SRA database and BLAST search against AtMAPK genes and determing conserved domain using a series of bioinformatics tools. There exist 16 MAPK genes contained T[D/E]Y conserved domains among the obtained genes. The expressions of these genes were analyzed by Real-time PCR under cadmium stress. The results showed that the expressions level of AaMAPK3 and AaMAPK10 were down-regulated and MAPK7, MAPK9 and MAPK12 were up-regulated. These indicated that there exist corresponding MAPK genes involved in transduction of the cadmium signal.