Preparation and identification of the oligonucleotide chip for detecting the virulence-associated genotypes and drug resistance of Helicobacter pylori.
- Author:
Shu-Bin GUO
1
;
Jun-Bo LIU
;
Lin-Jie CHEN
;
Sheng-Qi WANG
Author Information
- Publication Type:Journal Article
- MeSH: Anti-Bacterial Agents; pharmacology; Clarithromycin; pharmacology; Drug Resistance, Bacterial; Genotype; Helicobacter pylori; drug effects; genetics; pathogenicity; In Vitro Techniques; Microbial Sensitivity Tests; Oligonucleotide Array Sequence Analysis; Point Mutation; Polymerase Chain Reaction; Virulence
- From: Acta Academiae Medicinae Sinicae 2007;29(1):98-102
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop an oligonucleotide microarray-based method for the simultaneous detection of Helicobacter pylori (Hp) infection, virulence-associated genotypes, and drug resistance.
METHODSHp was classified into cagA + and cagA- genotypes based on its virulence. Clarithromycin-resistance of Hp was identified by existence of point mutations in 23S rRNA. We constructed an oligonucleotide microarray chip to simultaneously diagnose Hp infection and detect its virulence-associated genotypes and mutations associated with clarithromycin-resistance. The diagnostic accuracy of the constructed microarray was tested with templates of wild type and mutated type.
RESULTSThe oligonucleotide chip accurately detected cagA + and cagA- genotypes of Hp, as well as four common point mutations in 23S rRNA related to clarithromycin-resistance.
CONCLUSIONOligonucleotide microarray chip can be used to diagnose Hp infection and test its virulence-associated genotypes and drug resistance simultaneously.
