Effects of triptolide on the production of interferon-gamma in human peripheral blood mononuclear cell and phosphorylation of signal transducer and activator of transcription-1 and production of interleukin-8.
- Author:
Xin-Yu LI
1
;
Zhi-Yu QIAN
;
Ji-Wei GAO
;
Jia-Run ZHENG
Author Information
- Publication Type:Journal Article
- MeSH: Anti-Inflammatory Agents, Non-Steroidal; pharmacology; Diterpenes; pharmacology; Epoxy Compounds; pharmacology; Humans; Interferon-gamma; biosynthesis; pharmacology; Interleukin-8; biosynthesis; Keratinocytes; drug effects; metabolism; Leukocytes, Mononuclear; drug effects; metabolism; Phenanthrenes; pharmacology; Phosphorylation; STAT1 Transcription Factor; metabolism
- From: Acta Academiae Medicinae Sinicae 2007;29(2):158-162
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of triptolide on the production of interferon-gamma (IFN-gamma) in human peripheral blood mononuclear cell (PBMC) and interleukin-8 (IL-8) in HaCaT keratinocytes and phosphorylation of signal transducer and activator of transcription-1 (STAT1) of IFN-gamma signal transduction pathways in HaCaT cells.
METHODSHuman PBMC was induced by phytohaemagglutinin (PHA-L) and HaCaT cells were stimulated by recombinant human IFN-gamma (rhIFN-gamma). The productions of IFN-gamma and IL-8 in cells were detected by ELISA. The expression of STAT1 and its phosphorylation were analyzed by Western blot.
RESULTSTriptolide inhibited the production of IFN-gamma in human PBMC induced by PHA-L in a dose-dependent manner (P < 0.05, P < 0.01, P < 0.001) and the 50% inhibitory concentration (IC50) value was 5.96 x 10(-11) mol/L. IL-8 production in HaCaT cells induced by rhIFN-gamma in vitro was also inhibited by triptolide (P < 0.001) and the IC50 value was about 1.15 x 10(-13) mol/L. The expressions of phosphorylated STAT1 in HaCaT cells stimulated by rhIFN-gamma was inhibited by triptolide (P < 0.01) and the IC50 value was about 9.45 x 10(-11) mol/L.
CONCLUSIONTriptolide can inhibit the production of IFN-gamma in human PBMC and downregulate IL-8 level in HaCaT keratinocytes induced by rhIFN-gamma. Triptolide can inhibit the phosphorylations of STAT1 of IFN-gamma signal pathway in HaCaT keratinocytes stimulated by IFN-gamma.
