Genotyping of genital human papillomavirus by DNA sequencing and luminex methods.
- Author:
Shu-Zhen QI
1
;
Qian-Qiu WANG
;
Yu TAN
;
Yan SHEN
;
Bo LI
;
Shu-Li CHEN
;
Gang CHENG
;
Hong-You QIN
;
Zhi-Xue YOU
;
Bing-Bin ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Base Sequence; DNA, Viral; genetics; Female; Female Urogenital Diseases; virology; Genotype; Humans; Oligonucleotide Array Sequence Analysis; Papillomaviridae; genetics; isolation & purification; Papillomavirus Infections; virology; Polymerase Chain Reaction
- From: Acta Academiae Medicinae Sinicae 2007;29(2):181-185
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare the specificity and sensitivity of two genotyping approaches for human papillomavirus (HPV).
METHODHPV DNA was amplified and detected in clinical specimens by polymerase chain reaction in a pair of universal primers MY09/11, and then genotyped with either sequencing method or liquid chip hybridization method (luminex method).
RESULTSequencing method obtained precise genotyping results in single-type HPV infection, while luminex method obtained accurate genotyping results in multiple-type HPV infection.
CONCLUSIONA combined method using both sequencing and luminex method is suitable for the genotyping of HPV-infected specimens.
