Effects of antisense epidermal growth factor receptor oligodeoxynucleotides on ultraviolet-induced c-jun activity of keratinocytes.
- Author:
Ji-Ping XIA
1
;
Xiu-Zu SONG
;
Wei-Ling SUN
;
Yan-Hua LI
;
Jian KANG
;
Wen-Qi CHEN
;
Zhi-Gang BI
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line; Humans; JNK Mitogen-Activated Protein Kinases; metabolism; Keratinocytes; drug effects; metabolism; radiation effects; Oligodeoxyribonucleotides, Antisense; pharmacology; Receptor, Epidermal Growth Factor; biosynthesis; genetics; Transfection; Ultraviolet Rays
- From: Acta Academiae Medicinae Sinicae 2007;29(2):213-216
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effects of antisense epidermal growth factor receptor (EGF-R) oligodeoxynucleotides on ultraviolet-induced c-jun activity of keratinocytes after EGF-R oligodeoxynucleotides transfect to HaCaT in vitro.
METHODSc-jun DNA binding activity after ultraviolet-B (UVB) irradiation and EGF-R oligodeoxynucleotides transfection were determined with a highly sensitive and specific colorimetric method. After EGF-R oligodeoxynucleotides transfection, the mRNA level of EGF-R was detected by reverse transcription polymerase chain reaction method.
RESULTSCompared with control groups, c-jun activity increased significantly in UVB (10, 20, 30 mJ/cm2) irradiation groups (P < 0.05). EGF-R mRNA and c-jun activities induced by UVB were inhibited after the keratinocytes were transfected with EGF-R antisense oligodeoxynucleotides at 2, 4 and 8 microg/ml concentrations (P < 0.01).
CONCLUSIONThe ultraviolet-induced c-jun activity of keratinocytes can be mediated by EGF-R and inhibited by EGF-R antisense oligodeoxynucleotides, which is transfected to keratinocytes and mediated by lipofectamine.
