Genetic diagnosis of polycystic kidney disease using single cell fluorescent polymerase chain reaction.
- Author:
Jin HUANG
1
;
Ping LIU
;
Yong-Jian CHEN
;
Hong ZHANG
;
Dan-Xia ZHENG
;
Xiao-Wei ZHANG
;
Jie QIAO
Author Information
- Publication Type:Journal Article
- MeSH: Female; Fluorescent Dyes; Humans; Molecular Diagnostic Techniques; methods; Pedigree; Polycystic Kidney Diseases; diagnosis; genetics; Polymerase Chain Reaction; methods; Pregnancy; Preimplantation Diagnosis; methods
- From: Acta Academiae Medicinae Sinicae 2007;29(2):283-286
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the feasibility of using single cell fluorescent polymerase chain reaction (PCR) in the preimplantation genetic diagnosis (PGD).
METHODSingle buccal cell genetic analysis was performed with fluorescent PCR of linked microsatellite D16S423, followed by electrophoresis on ABI 3730.
RESULTThe amplification success rate, allele dropout rate, and diagnostic accuracy rate of the single cell fluorescent PCR were 93.3%, 10.7%, and 89.3%, respectively.
CONCLUSIONSingle cell fluorescent PCR is a stable and reliable approach for the PGD.
