Identification of binding proteins to the PDZ domain of ERBIN.
- Author:
Chun-jiao SONG
1
;
De-xian ZHENG
;
Yan-xin LIU
Author Information
- Publication Type:Journal Article
- MeSH: Adaptor Proteins, Signal Transducing; metabolism; Cell Line, Tumor; Humans; Immunoprecipitation; Intracellular Signaling Peptides and Proteins; metabolism; Neoplasm Proteins; metabolism; PDZ Domains; Protein Binding; Two-Hybrid System Techniques
- From: Acta Academiae Medicinae Sinicae 2007;29(3):307-311
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo identify the binding proteins to PDZ domain of ERBIN.
METHODSUsing PDZ domain of ERBIN as the bait, yeast two-hybrid technology was employed to screen the human lymphocyte leukemia cells MATCHMAKER cDNA library. The protein interaction was identified by immunoprecipitation.
RESULTSA PDZ-binding protein, TAX1, was identified.
CONCLUSIONTAX1 is a novel binding protein to PDZ domain of ERBIN.
