Characteristics of a novel human testis-specific gene, HSD-9, and its encoding protein.
- Author:
Yong WANG
1
;
Yi-nan CHEN
;
Yuan QIAO
;
Shi-ying MIAO
;
Lin-fang WANG
Author Information
- Publication Type:Journal Article
- MeSH: Amino Acid Sequence; Animals; Base Sequence; Clathrin; metabolism; Humans; Male; Membrane Proteins; biosynthesis; genetics; Molecular Sequence Data; Organ Specificity; Rabbits; Rats; Testis; metabolism
- From: Acta Academiae Medicinae Sinicae 2007;29(3):318-323
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the characteristics of a novel human testis-specific gene, HSD-9, and its encoding protein.
METHODSHSD-9 was a novel gene from a human germ cells-specific ESTs library established in our laboratory. We used an electronic cloning method to obtain HSD-9 gene, and analyzed the characteristics of this novel gene and encoding product by bioinformatics methods, detected its expressing profile using a Northern blot assay, prepared specific rabbit polyclonal antibodies against HSD-9 protein, observed the localization of this protein in the germ cells and some somatic cells with confocal microscopy.
RESULTSHSD-9 was expressed in human testes, and its rat homolog was found in the varying germ cells. HSD-9 protein could partly be colocalized with clathrin.
CONCLUSIONSHSD-9 is specific in human testes, and the expression pattern of its encoding product is similar to those of some endocytosis proteins. It is speculated that HSD-9 protein may function in the endocytosis.
