Effects of oxidative stress on the expression of endothelial-leukocyte adhesion molecule-1 in porcine trabecular meshwork cells.
- Author:
Qi ZHOU
1
;
Yu-qin LIU
;
Jia-liang ZHAO
;
Hua ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cells, Cultured; E-Selectin; biosynthesis; Hydrogen Peroxide; pharmacology; Interleukin 1 Receptor Antagonist Protein; pharmacology; Oxidative Stress; Swine; Trabecular Meshwork; metabolism
- From: Acta Academiae Medicinae Sinicae 2007;29(3):394-397
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of oxidative stress on the expression of endothelialleukocyte adhesion molecule-1 (ELAM-1) in porcine trabecular meshwork cells and to elucidate the relationship between the expression of ELAM-1 and IL-1alpha.
METHODSPrimary cultured porcine trabecular meshwork cells were cultured without serum and treated with 1 mmol/L H2O2 with or without pretreatment of IL-1ralpha of different concentrations. The expression of ELAM-1 was tested by immunocytochemistry.
RESULTSELAM-1 immunocytochemistry staining was positive in the H2O2-treated porcine trabecular meshwork cells. It was negative in the porcine trabecular meshwork cells pretreated with IL-1alpha of high concentrations (180 microg/ml and 600 microg/ml).
CONCLUSIONSOxidative stress can induce the expression of ELAM-1 in porcine trabecular meshwork cells. In the in vitro cell system, IL-1ralpha of high concentration can inhibit the expression of ELAM-1 induced by oxidative stress.
