High mobility group box-1 protein induces apoptosis in mouse peritoneal macrophages.
- Author:
Feng LIU
1
;
Yong-ming YAO
;
Ning DONG
;
Shan XU
;
Zhi-yong SHENG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; Female; HMGB1 Protein; physiology; In Vitro Techniques; Macrophages, Peritoneal; cytology; metabolism; Mice; Mice, Inbred BALB C
- From: Acta Academiae Medicinae Sinicae 2007;29(4):488-492
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of high mobility group box-1 protein (HMGB1) on apoptosis of macrophages in mice.
METHODSThe peritoneal macrophages were obtained from female BALB/c mice. After exposure to different doses of HMGB1 (10, 100, 1000, and 10000 ng/ml) for 24 hours, or exposure to 10 000 ng/ml for different time (6, 12, 24, and 48 hours), cells were stained with Annexin V-PE and 7-amino-actinomycin-D (7-AAD), then determined by flow cytometry and laser scanning confocal microscopy, respectively.
RESULTSExposed to HMGB1 for 24 hours resulted in a dose-dependent induction of apoptosis, with increased levels of apoptosis observed at 100, 1 000, and 10 000 ng/ml compared with controls [(12.91 +/- 3.89)%, (18.76 +/- 3.41)%, and (39.84 +/- 5.98)%, respectively, vs. (4.49 +/- 1.72)%]. Exposed to HMGB1 at 10000 ng/ml for 6, 12, 24, or 48 hours showed a time-dependent increase in apoptosis rate [(18.33 +/- 4.60)%, (25.02 +/- 5.27)%, (38.30 +/- 6.95)%, and (21.81 +/- 7.96)%]. The percentage of apoptotic cells markedly increased with a peak value at 24 hours after HMGB1 stimulation (P<0.01).
CONCLUSIONHMGB1 has a proapoptotic effect on murine peritoneal macrophages.
