Effects of the box-3 region of the LIFRalpha-chain cytoplasmic domain (gp190CT3) on the proliferation and differentiation of HL-60 cells.
- Author:
Ling YANG
1
;
Shan-rong LIU
;
Shu-ping TANG
;
Feng-mei WANG
;
Hou-qi LIU
Author Information
- Publication Type:Journal Article
- MeSH: Binding Sites; genetics; Blotting, Western; Cell Differentiation; genetics; physiology; Cell Proliferation; Genetic Vectors; genetics; HL-60 Cells; Humans; Immunohistochemistry; Lewis X Antigen; metabolism; Proliferating Cell Nuclear Antigen; metabolism; Receptors, OSM-LIF; genetics; metabolism; Transfection
- From: Chinese Journal of Hematology 2004;25(11):679-682
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of Box-3 region of the leukemia inhibitory factor receptor (LIFR) alpha-chain cytoplasmic domain on the proliferation and differentiation of HL-60 cells.
METHODSExpression vector of gp190CT3 was constructed and expressed in HL-60 cells. The expression level of gp190CT3 was assayed by immunocytochemistry. The growth of wild type and gp190CT3 transfected HL-60 cells were examined under microscope. The PCNA levels were assayed by Western blot, and the levels of CD15 by flow cytometry.
RESULTSThe gp190CT3 transfected HL-60 cells were enlarged in size and their proliferation was slower than that of wild type. The expression level of PCNA was down-regulated while the level of CD15 up-regulated in transfected HL-60 cells as compared with that of the wild type cells.
CONCLUSIONThe Box-3 region of the leukemia inhibitory factor receptor alpha-chain cytoplasmic domain (gp190CT3) participates the LIFR signal transduction in inhibiting the growth and inducing the differentiation of HL-60 cells.
