Cytomegalovirus infection of myeloma cell line KM3 and its effect on IL-6 mRNA expression in the cells.
- Author:
Guo-qing WEI
1
;
Mao-fang LIN
;
He HUANG
;
Zhen CAI
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Cytomegalovirus; genetics; physiology; ultrastructure; Gene Expression Regulation, Neoplastic; Host-Pathogen Interactions; Humans; Immediate-Early Proteins; genetics; Interleukin-6; genetics; Microscopy, Electron, Transmission; Multiple Myeloma; genetics; pathology; virology; RNA, Messenger; genetics; metabolism; Reverse Transcriptase Polymerase Chain Reaction; Viral Proteins; genetics
- From: Chinese Journal of Hematology 2004;25(10):596-599
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the infection of myeloma cell line KM3 by cytomegalovirus (CMV) as well as its effect on IL-6 mRNA in the cells.
METHODSRT-PCR assay was used to detect the mRNA expression of CMV immediate early gene (IE), glyceraldehyde phosphate dehydrogenase (GAPDH) and IL-6 of KM3 cells infected by 100-, 10-, 1-folds of median tissue culture infective dose (TCID(50)) of CMV. Flow cytometry was used to detect the expression of pp65 antigen. CMV particles were detected with electron microscope.
RESULTSKM3 cells infected by CMV could express IE mRNA as compared with the uninfected cells. CMV pp65 antigen positive cells were (5.58 +/- 1.55)% and (3.75 +/- 0.85)% respectively when infected by 100 and 10 TCID(50) of CMV, which were significantly higher than that of control group (P < 0.05). CMV particles could be seen in the infected cells. Expression of IL-6 mRNA was increased after the infection.
CONCLUSIONCMV can infect myeloma cell line KM3 and replicate in the cells. CMV can increase the expression of IL-6 mRNA.
