Differential expression of a homing-related molecule repertoire among umbilical cord blood, mobilized peripheral blood and bone marrow-derived hematopoietic stem/progenitor cells.
- Author:
Yi-zhou ZHENG
1
;
Li ZHANG
;
Hui-jun WANG
;
Zhong-chao HAN
;
Tsuneo A TAKAHASHI
Author Information
- Publication Type:Journal Article
- MeSH: Antigens, CD34; immunology; Bone Marrow Cells; cytology; immunology; metabolism; CD11a Antigen; immunology; CD18 Antigens; immunology; Cell Adhesion Molecules; metabolism; Cells, Cultured; Fetal Blood; cytology; Flow Cytometry; Hematopoietic Stem Cell Mobilization; Hematopoietic Stem Cells; cytology; immunology; metabolism; Humans; Hyaluronan Receptors; immunology; Infant, Newborn; Matrix Metalloproteinases, Secreted; metabolism; Platelet Endothelial Cell Adhesion Molecule-1; immunology; Receptors, CXCR4; metabolism
- From: Chinese Journal of Hematology 2004;25(12):736-739
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare the expression profiles of a set of homing-related molecules (HRM) repertoire expressed on hematopoietic stem/progenitor cells (HS/PC) from different sources.
METHODThe expression levels of HRM on HS/PC from umbilical cord blood (UCB), mobilized peripheral blood (mPB) and bone marrow (BM) were assessed using a highly sensitive 4-color flow cytometric analysis.
RESULTSUCB-derived CD34(bright) cells, as well as mPB- and BM-derived CD34(bright) cells strongly expressed CD44, CD11a, CD18, CD62L, CD31 and CD49d. On the other hand, significantly lower expressions of CD49e, CD49f, CXCR-4 and CD54 on UCB-derived CD34(bright) and CD34(bright)CD38(-) cells, compared with those on mPB- and BM-derived CD34(bright) and CD34(bright)CD38(-) cells, were observed. None of UCB-, mPB- and BM-derived CD34(bright) cells expressed other chemokine receptors, including CCR-1, CCR-2, CCR-3, CCR-5, CXCR-1, CXCR-2, CXCR-3 and CXCR-5. Another striking finding was that only mPB-derived CD34(bright) cells expressed significant levels of both the matrix metalloproteinases MMP-2 \[(11.4 +/- 4.9)%\] and MMP-9 \[(27.6 +/- 7.8)%\].
CONCLUSIONHS/PC from UCB have some defects of expression of HRM repertoire, which might partly explain the cause(s) of delayed hematopoietic reconstitution after UCB transplant.
