Differentiation of rat bone marrow derived Thy-1+ beta2M- cells into hepatocytes.
- Author:
Yun-fang WANG
1
;
Xue NAN
;
Yan-hua LI
;
Rui ZHANG
;
Fang YAN
;
Wen YUE
;
Xue-tao PEI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bone Marrow Cells; cytology; metabolism; Cell Differentiation; Cells, Cultured; Coculture Techniques; Fibroblast Growth Factor 2; pharmacology; Gene Expression; drug effects; Hepatocyte Growth Factor; pharmacology; Hepatocytes; cytology; drug effects; metabolism; Immunohistochemistry; Immunomagnetic Separation; Keratin-18; biosynthesis; genetics; Propanols; pharmacology; Rats; Rats, Wistar; Reverse Transcriptase Polymerase Chain Reaction; Thy-1 Antigens; metabolism; alpha-Fetoproteins; biosynthesis; genetics
- From: Chinese Journal of Hematology 2005;26(2):69-73
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the differentiation of bone marrow derived Thy-1(+)beta(2)M(-) cells (BDTC) into mature and functional liver cells and its mechanism.
METHODSBDTC were cocultured with allyl alcohol (AA)-injured hepatocytes and cultured alone in conditional medium containing HGF and bFGF, respectively. BDTC morphologic transformation was observed with phase-contrast and electron-microscopy. Hepatocyte-specific gene expression in cultured BDTC was identified by immunocytochemistry and reverse transcription-polymerase chain reaction (RT-PCR). Indocyanine green (ICG) ingestion/excretion and urea, albumin production were carried out to evaluate hepatocyte-related function.
RESULTSSome BDTC derived hepatocyte-like cells with high nuclear to cytoplasmic ratio containing mono- or multi-nuclei and abundant mitochondria, endoplasmic reticulum and glycogenic granules appeared after 7-day culture in both the two culture systems. These cells expressed hepatocyte-specific genes (AFP, OV-6, CK18, etc.), and possessed functions of ICG uptake, albumin production and ammonium metabolism.
CONCLUSIONRat BDTCs could differentiate into mature and functional liver cells in special stimulation systems. Moreover, these differentiations were realized by "transdifferentiation", and might dispense with "cell fusion".
