Clonal study on single apoptotic cells in myelodysplastic syndromes.
- Author:
Xiao LI
1
;
Wei-ming SHEN
;
Quan PU
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Aged, 80 and over; Apoptosis; Bone Marrow Cells; metabolism; pathology; Cells, Cultured; Clone Cells; metabolism; pathology; Female; Flow Cytometry; Humans; In Situ Hybridization, Fluorescence; In Situ Nick-End Labeling; Karyotyping; Male; Middle Aged; Myelodysplastic Syndromes; genetics; metabolism; pathology; fas Receptor; metabolism
- From: Chinese Journal of Hematology 2005;26(1):39-43
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the clonal origin of the apoptotic hematopoietic cells in myelodysplastic syndromes (MDS).
METHODSNineteen patients with MDS whose karyotypes had been analysed with G- or R-binding were studied. ISEL (DNA in situ end labelling) and FISH (fluorescence in situ hybridization) (simplified as ISEL/FISH) were performed simultaneously in a single nucleated cells on cytospin slides in 10/19 of the patients. The ratio of FISH positive cells to all nucleated cells and to apoptotic cells were counted. Eight slides from 4 normal donors induced apoptosis by Fas monoclonal antibody (McAb) were used as methodology control. For the rest 9 patients, different fractions of cells (PI-, Annexin V+PI-, Annexin V-PI-) were sorted by flow cytometry and then cytospined for FISH analysis (simplified as FCM/FISH).
RESULTSThe mean percentage of abnormal clonal cells in nuclear cells in the 10 cases detected by ISEL/FISH was 37.1%, whereas it was 24.0% in apoptotic cells. Induction by Fas McAb didn't lead to change of karyotype. Moreover, 8/9 patients analysed by FCM/FISH showed higher cell proportion with normal karyotypes in apoptosis-undergoing cells than that in non-apoptotic cells.
CONCLUSIONOver apoptotic hematopoietic cells in MDS are mainly residual normal hematopoietic cells.