Study on the direct MAIPA technique in the differential diagnosis of immune and non-immune thrombocytopenia.
- Author:
Ping QIN
1
;
Ming HOU
;
Jian-zhi SUN
;
Lu LU
;
Yan SHI
;
Yuan-yuan ZHU
;
Li-zhen LI
;
Mao-hong ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Aged; Antibodies, Monoclonal; immunology; Autoantibodies; blood; Diagnosis, Differential; Female; Humans; Male; Middle Aged; Platelet Membrane Glycoproteins; immunology; Purpura, Thrombocytopenic; diagnosis; immunology; Young Adult
- From: Chinese Journal of Hematology 2005;26(3):167-169
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the clinical usefulness of direct monoclonal antibody immobilization of platelet antigen (MAIPA) technique in the differential diagnosis of immune and non-immune thrombocytopenia.
METHODSPlatelet-bound autoantibodies in thrombocytopenic patients (immune and non-immune) were measured by direct MAIPA. Monoclonal antibodies against GP II b/III a, GPIb and GP I a/II a were used.
RESULTSThe positive rates of platelet-bound GP-specific autoantibodies between immune (76.4%) and non-immune thrombocytopenia (3.6%) were significantly different (P < 0.05). The direct MAIPA had a sensitivity of 76.4%, a specificity of 96.4%, and a positive predictive value of 97.1% for the diagnosis of immune thrombocytopenia. There was a significant inverse correlation between platelet-bound GP II b/III a specific autoantibody levels and platelet counts (r = -0.338, P < 0.05).
CONCLUSIONThe direct MAIPA technique can be used to differentiate immune from non-immune thrombocytopenias.
