The effects of chloride channel blockers on thrombocytic cytoplasmic free calcium concentration and platelet aggregation.
- Author:
Song-Mei YIN
1
;
Xiao-Lin CHEN
;
Da-Nian NIE
;
Shuang-feng XIE
;
Li-ping MA
;
Xiu-ju WANG
;
Yu-dan WU
;
Yi-qing LI
;
Jian-hong FENG
Author Information
- Publication Type:Journal Article
- MeSH: 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; pharmacology; Adult; Blood Platelets; cytology; drug effects; metabolism; Calcium; metabolism; Calcium Channel Blockers; pharmacology; Cells, Cultured; Chloride Channels; antagonists & inhibitors; physiology; Cytoplasm; drug effects; metabolism; Drug Interactions; Humans; Imidazoles; pharmacology; Nifedipine; pharmacology; Niflumic Acid; pharmacology; Platelet Aggregation; drug effects; Thrombin; pharmacology
- From: Chinese Journal of Hematology 2005;26(3):170-174
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effects of chloride channels on the regulation of platelet cytoplasmic free calcium concentration ([Ca2+]i) and platelet aggregation (PAG).
METHODSFreshly separated platelets were activated by thrombin. Chloride channel blockers DIDS or NFA and calcium channel blockers SK&F96365 or nifedipine were added to study the effects on platelet [Ca2+]i and PAG by a single reagent or the combination of reagents and find out the interactions among DIDS, NFA, SK&F96365 and nifedipine.
RESULTSBoth DIDS and NFA could inhibit the thrombin (1 U/ml) induced PAG in a dose-dependent manner, whereas had little effect on resting [Ca2+]i. As compared with the control group, DIDS, SK&F96365 and Nifedipine could significantly reduce the PAG, Ca2+ release and Ca2+ influx in thrombin activated platelet (P < 0.05). The combination of DIDS and SK&F96365 had greater effects in reducing the PAG, Ca2+ release and Ca2+ influx than either reagent alone (P < 0.05). The combination of DIDS and nifedipine also had greater effect than each alone in reducing Ca2+ release (P < 0.05). The combination of NFA and SK&F96365 weakened each other's effect on Ca2+ release (P < 0.05), while NFA and nifedipine weakened each other's effects on PAG, Ca2+ release and Ca2+ influx in thrombin activated platelet (P < 0.05).
CONCLUSIONDIDS and NFA have no effect on the resting [Ca2+]i and the leak calcium influx of platelet. DIDS can inhibit the Ca2+ release, Ca2+ influx and PAG of platelet induced by thrombin, while NFA can only inhibit the Ca2+ release. The chloride channel and calcium channel blockers have interactions in affecting resting [Ca2+]i and PAG of platelet. The opening of chloride channel can influence the cellular calcium movement of platelet.
