Effect of 2-methoxyestradiol on cell differentiation of myeloma cell line CZ-1.
- Author:
Wei-Ran GAO
1
;
Jian HOU
Author Information
- Publication Type:Journal Article
- MeSH: Cell Differentiation; drug effects; Cell Line, Tumor; Dose-Response Relationship, Drug; Estradiol; analogs & derivatives; pharmacology; Flow Cytometry; Humans; Integrin alpha5; analysis; Multiple Myeloma; metabolism; pathology; Tubulin Modulators; pharmacology
- From: Chinese Journal of Hematology 2005;26(4):197-199
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the differentiation induction effect of 2-methoxyestradiol (2ME2), an estrogen derivative on myeloma cell line CZ-1.
METHODSThe changes of CZ-1 cells in morphology, expression of surface CD49e and quantity of light chain secretion in the supernatant were observed when treated with 0.1 approximately 0.5 micromol/L 2ME2 for 48 h.
RESULTS2ME2 could induce differentiation of CZ-1 cells. The cells appeared decreased in size of nucleus, increased in cytoplasma, decreased in the ratio of nucleus to plasma, decreased in number or disappearance of nucleolus, and thickness and pyknosis of chromatin. The expression of CD49e was increased from (12.20 +/- 1.57)% to (24.80 +/- 1.26)% (P < 0.05). Light chain secretion in the supernatant was increased from (35.97 +/- 2.60) microg/ml to (79.67 +/- 1.88) microg/ml (P < 0.05).
CONCLUSIONLow concentrations of 2ME2 could induce differentiation of myeloma cell line CZ-1.
