The methylation locus and frequency pattern on p16 INK4a gene promoter CpG in epidermis of patients with psoriasis.
- Author:
Min CHEN
1
;
Pan-gen CUI
;
Xu YAO
;
Yuan-hua CAO
;
Juan-qin GONG
;
An-sheng LI
;
Zhi-qiang CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Aged; Base Sequence; CpG Islands; genetics; Cyclin-Dependent Kinase Inhibitor p16; genetics; metabolism; DNA Methylation; genetics; Epidermis; metabolism; Female; Humans; Male; Middle Aged; Molecular Sequence Data; Polymerase Chain Reaction; Promoter Regions, Genetic; genetics; Psoriasis; genetics; Sequence Alignment; Tumor Suppressor Protein p14ARF; genetics; Young Adult
- From: Chinese Journal of Medical Genetics 2007;24(6):674-676
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the CpG methylation locus and frequency pattern on p16 INK4a gene promoter in epidermis of p16 INK4a methylated patients with psoriasis vulgaris.
METHODSThe DNA specimens were obtained from epidermal lesion of 50 plaque psoriatic patients. Methylation specific PCR and DNA sequencing were used to detect the frequency and locus of methylation in p16 INK4a gene promoter region.
RESULTSApproximately 50% CpG was methylated in p16 INK4a methylated patients, methylation was found in specifical locus of p16 INK4a gene promoter.
CONCLUSIONThe distinct methylation pattern is showed on the p16 INK4a gene promoter region in patients with psoriasis.
