Analysis of chromosome mosaicism in preimplantation embryos by using 2 sequential rounds of fluorescence in situ hybridization.
- Author:
Xiu-lian REN
1
;
Yan-wen XU
;
Can-quan ZHOU
;
Guang-lun ZHUANG
Author Information
- Publication Type:Journal Article
- MeSH: Aneuploidy; Blastocyst; Chromosomes, Human; Embryo Transfer; Female; Humans; In Situ Hybridization, Fluorescence; methods; Mosaicism; chemically induced; embryology; Preimplantation Diagnosis
- From: Chinese Journal of Medical Genetics 2007;24(6):706-708
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the mechanism and factors affecting mosaicism in human preimplantation embryos by using 2 sequential rounds of fluorescence in situ hybridization(FISH).
METHODSTotally 51 normal fertilized embryos, which were not suitable for embryo transfer and cryopreservation, were analyzed on day 3 after fertilization by using two sequential rounds of FISH. Chromosomes 13, 16, 18, 21, 22, X and Y were analyzed.
RESULTSAmong 51 embryos, 16 (31.4%) were mosaic, 12 (23.5%) were chaotic, and the remaining were either normal (27.5%) or non-mosaic abnormal (17.6%). The incidence of mosaic embryos was related to embryo developmental stage, for the incidence of mosaicism increased from 12.5% in embryos
CONCLUSIONMosaicism is common in human preimplantaion embryos, which may be one of the important factors affecting the success rates in IVF-ET. Most of the chromosomal abnormalities can be identified by two sequential rounds of FISH.
