The building of chip-based capillary electrophoresis platform and application.
- Author:
Hua HU
1
;
Qiang XIONG
;
Cai-xia LI
;
Hua-fang GAO
;
Qian YANG
;
Zhi-qing LIANG
Author Information
- Publication Type:Journal Article
- MeSH: Electrophoresis, Capillary; methods; Female; Genetic Diseases, Inborn; diagnosis; genetics; Humans; Male; Oligonucleotide Array Sequence Analysis; methods; beta-Thalassemia; diagnosis; genetics
- From: Chinese Journal of Medical Genetics 2007;24(6):709-712
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect the point mutations by chip-based capillary electrophoresis and to provide a rapid and sensitive technique detection for beta-thalassemia.
METHODSMultiplex primer-extension reaction was used to amplify the common loci of the samples for beta-thalassemia. The reaction products were detected by the chip-based capillary electrophoresis and the genotypes of the samples were discrened.
RESULTSA system was constructed to detect the point mutations of beta-thalassemia by chip-based capillary electrophoresis, and the technology was ralidated by the patients' samples and the results coincided with those of detection kit.
CONCLUSIONBeta-thalassemia can be detected by chip-based capillary electrophoresis rapidly with a small amount of samples. It would be a new detection method of the genetic disorders.