Detection of Y chromosome microdeletions in AZF region by liquid chip technology.
- Author:
Si-yao LIU
1
;
Xian-ping DING
;
Xia WEI
;
Ping WEI
;
Hai-rong PAN
Author Information
- Publication Type:Journal Article
- MeSH: Asian Continental Ancestry Group; genetics; Azoospermia; genetics; Base Sequence; Chromosome Deletion; Chromosomes, Human, Y; genetics; Electrophoresis, Polyacrylamide Gel; Humans; Male; Oligonucleotide Array Sequence Analysis; methods; Oligospermia; genetics; Sequence Tagged Sites
- From: Chinese Journal of Medical Genetics 2008;25(1):86-88
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a liquid chip technology to detect Y chromosome microdeletions in Chinese infertile males with azoospermia or oligozoospermia.
METHODSMultiplex PCR and liquid chip technology were used to detect the Y chromosome microdeletions in AZF region in 178 infertile patients with azoospermia and 134 infertile patients with oligozoospermia as well as 40 fertile control men.
RESULTSForty out of 312 patients (12.8%) were found to have deletions in AZF region. The microdeletion frequency was 14%(25/178) in the azoospermic group, 9.6%(11/114) in the oligospermic and 20%(4/20) in the severe oligospermic group.
CONCLUSIONThe authors developed a high-throughput, fast and simple assay to screen the AZF region microdeletions of Y chromosome.
