Study on the genetic stability of immortalized cell line of lymphocyte cell transformed by EB virus after long subculture process.
- Author:
Yan-han LI
1
;
Xiao-qin HUANG
;
Ke-qin LIN
;
Yu-fen TAO
;
Wen YI
;
Yu-feng YAO
;
Lei SHI
;
Jia-you CHU
Author Information
- Publication Type:Journal Article
- MeSH: Cell Transformation, Viral; genetics; Herpesvirus 4, Human; genetics; Humans; Lymphocytes; cytology; metabolism; virology; Microsatellite Repeats; genetics; Polymerase Chain Reaction
- From:Chinese Journal of Medical Genetics 2008;25(3):276-279
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the genetic stability of an immortalized cell line transformed by Epstein-Barr virus (EBV) after long subculture process.
METHODSIn the present study, the genetic stability including chromosome diploidy, karyotypes and microsatellite DNA were evaluated with chromosome banding techniques and microsatellite DNA detection. The telomerase activity of the immortalized cell line was detected by using the telomerase assay kit.
RESULTSFrom passage 1 to 30, there were no change of the diploidy, karyotypes of chromosome and microsatellite DNA, and the telomerase activity is negative.
CONCLUSIONThis study indicates that the immortalized cell line remains stable genetically within limited passages.
